pGLO Lab

Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB about 20 about 20 none - pGLO LB/amp none none none + pGLO LB/amp about 20 about 20 none + pGLO LB/amp/ara at least 50 at least 60 at least 40

The transformed bacteria glow and have grown into colonies. I estimate about 70 colonies of bacteria were on each plate. I think this because the glowing bacteria had about 50 colonies, and there were more colonies without the UV light that had grown. The arabinose in the plates allows the bacteria to glow. The only plate with arabinose was the one plate of bacteria that glowed green. GFP is used to make animals glow green, as scientists have done recently. It is also used to make biosensors, which sense the pH or ion level in something and display the results in what color the protein glows. Scientists have used GFP to turn cancer cells a bright, glowing green, allowing them to see where the cancer cells spread through an animal with translucent skin, like frogs. Genetic engineering is used to test which certain genes in organism have what effects or outcomes.